nucleic acid testing for the detection of hbv dna

author’s reply; nucleic acid testing for the detection of hbv dna

Author’s Reply; Nucleic Acid Testing for the Detection of HBV DNA

First, we agree with Seto et al. regarding the impacts of the sensitivity of the detection methods of OBI (occult hepatitis B infection) prevalence but disagree regarding their idea on the lack of usefulness of anti-HBc screens. They have referred to Seo et al., who reported that the prevalence of anti-HBc positivity in Korea was 13.5%, whereas the prevalence of OBI was 0.016% (1). Anti-HBc is ...

Nucleic acid testing to detect HBV infection in blood donors.

BACKGROUND The detection of hepatitis B virus (HBV) in blood donors is achieved by screening for hepatitis B surface antigen (HBsAg) and for antibodies against hepatitis B core antigen (anti-HBc). However, donors who are positive for HBV DNA are currently not identified during the window period before seroconversion. The current use of nucleic acid testing for detection of the human immunodefic...

Detection of HBV DNA in HBsAg Negative Normal Blood Donors

Background: The risk of infection by transfusion-transmitted viruses has been reduced remarkably. However, a zero-risk blood supply is still desirable. The screening for antibody to HBc (anti-HBc) has been shown as an alternative test for the detection of HBV infection. Objective: The main aim of this study was to evaluate HBV infection markers and the potential value of anti-HBc testing of blo...

Nucleic acid amplification strategies for DNA microarray-based pathogen detection.

DNA microarray-based screening and diagnostic technologies have long promised comprehensive testing capabilities. However, the potential of these powerful tools has been limited by front-end target-specific nucleic acid amplification. Despite the sensitivity and specificity associated with PCR amplification, the inherent bias and limited throughput of this approach constrain the principal benef...

nucleic acid-based approaches for detection of viral hepatitis

conclusions despite rapidity, automation, accuracy, cost-effectiveness, high sensitivity, and high specificity of molecular techniques, each type of molecular technology has its own advantages and disadvantages. results various types of molecular biology tools can be used to detect and quantify viral genomic elements and analyze the sequences. these molecular assays are proper technologies for ...